The Mad City Labs' MicroMirror TIRF microscope is part of our RM21™ microscope family and draw on our expertise with high precision, high stability piezo nanopositioning systems and microscopy solutions. The microscopy technique of colocalization single molecule spectroscopy (CoSMoS) is the only proven method to study the ordered assembly and function of multicomponent biomolecular machines. The MicroMirror TIRF microscope is the only commercially available CoSMoS microscope.
The MicroMirror TIRF microscope uses through-the objective excitation, but replaces the dichroic used in conventional TIRF systems with two broadband micro-mirrors positioned at the back aperture of the objective lens. Spatially separating the excitation and emission beams and eliminating the dichroic mirror leads to superior signal-to-noise ratios and simplifies the introduction of multiple spectral lines to your experiment. Typical biomolecular systems studied using micro-mirror TIRF incorporate 3 or more laser lines which can be difficult to accommodate in conventional dichroic TIRF instruments. Each micro-mirror is mounted to a multi-axis precision mount allowing the user to make fine adjustments to the optical pathway. As with all RM21™ microscopes it is designed to maximize optical pathway accessibility and flexibility, while also simplifying the optical alignment.
- Integrated piezo nanopositioning system
- Integrated imaging & motion control platform
- Spatially separated excitation & emission beams
- Adjustable micromirrors
- Open access to optical pathways
- Highly stable, yet flexible, platform
- Integrated slide holder
- TIRF Lock feedback control