CHROMATOGRAPHIC GRADE™ FOR POST-COLUMN ANALYSIS OF SULFA DRUGS IN FEED AND ANIMAL TISSUES
p-Dimethylaminobenzaldehyde (DMAB; Ehrlich’s Reagent) reacts rapidly with many primary amines to form a complex with maximum absorbance at 450 nm.
Reversed-phase LC followed by post-column derivatization is used to determine the levels of Sulfamethazine, Sulfathiazole and related compounds in feeds, premixes and animal tissues. Although these compounds have a UV chromophore, so do most of the other constituents in the complex sample matrix. Forming a colored derivative postcolumn allows shifting of the detection wavelength into the visible range thus greatly enhancing selectivity.